Name: Proteinase K, 1 g
Brand: Carl Roth
SKU: 3726.2
Price: 1018.0 EUR
Availability: InStock

Proteinase K, 1 g

Product Details

P-phrases: P261 P280 P305+P351+P338

H-phrases: H319-H335

CAS number: 77-92-9

Chemical formulas: C6H8O7

HS code: 35079090

Brand: Carl Roth

Proteinase K, lyophil., min. 35 U/mg, BioScience-Grade, Storage temp. -20 °C, CAS No. [39450-01-6], EG-Nr. 254-457-8

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.

Gebruiksinstructies

Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg²⁺-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca²⁺-ions (1-5 mM) prevent autolysis.

Proteinase K ≥35 U/mg, BioScience Grade, lyophilised

Endopeptidase for the degradation of proteins with very high solubility, specific activity and DNA purity. Extra high purified.

Voordelen:

High solubility
High specific activity
Extremely low DNA content

Activity: ≥ 35 U/mg lyophilizate
Specific activity: ≥ 45 U/mg protein
DNA content: ≤ 0.1 pg/mg

Gebruiksinstructies

Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl₂; 0.5 % SDS

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

• Oxidoreductases (catalyse redox reactions)

• Transferases (transfer functional groups among substrates)

• Hydrolases (cleave bonds via addition of water)

• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

• Isomerases (transform chemical isomers)

• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)

Appearance	white powder
Activity	≥35 U/mg
Specific activity	≥45 U/mg
Solubility in H₂O	≥50 mg/ml
Assay DNA	≤0.1 pg/mg
Exonuclease activity	not detectable
Endonuclease activity	not detectable
Ribonucleases activity	not detectable