Name: Proteinase K - Solution, 1 ml
Brand: Carl Roth
SKU: 3719.1
Price: 54.18 EUR
Availability: InStock

Proteinase K - Solution, 1 ml

Product Details

P-phrases: P280 P302+P352 P305+P351+P338 P308+P313

H-phrases: H226-H312+H332-H319-H360D

CAS number: 68-12-2

Chemical formulas: C3H7NO

Hazard class: 3 III

HS code: 35079090

Brand: Carl Roth

Proteinase K - Solution, 20 mg/ml, sterile, ready-to-use, for biochemistry and moleculr biology, Density (D) 1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. -20 °C, CAS No. [39450-01-6], EG-Nr. 254-457-8

Steriel

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.

Gebruiksinstructies

Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg²⁺-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca²⁺-ions (1-5 mM) prevent autolysis.

Proteinase K - Solution 20 mg/ml, sterile, ready-to-use, for biochemistry and molecular biology

Sterile ready-to-use solution
Stable over a wide pH range: 4.0–12.5
Active at high temperatures and denaturing conditions

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

• Oxidoreductases (catalyse redox reactions)

• Transferases (transfer functional groups among substrates)

• Hydrolases (cleave bonds via addition of water)

• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

• Isomerases (transform chemical isomers)

• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)

Appearance	clear, colorless solution
Concentration	20 mg/ml
Specific activity	≥800 U/ml
Assay DNA	≤200 pg/ml
Exonuclease activity	not detectable
Endonucleases	not detectable