ROTI®Blue, 250 ml
SKU A152.2
€ 43,17
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ROTI®Blue, 250 ml
Product Details
CAS number: 56-40-6
Chemical formulas: C2H5NO2
HS code: 38229000
Brand: Carl Roth
ROTI®Blue, 5x concentrated, staining solution for protein gels, Density (D) 1,24 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 1805
ROTI®Blue 5x conc.
ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring.
- Most simple application
- No destaining necessary
- 5x more sensitive than Coomassie® R 250
Figures:
A: Horizontal IEF for determination of wheat-varieties.
With kind permission of R. Knoblauch, LTZ Referat 23, Karlsruhe
B: SDS-PAGE ROTI®Mark Standard.
A: Horizontal IEF for determination of wheat-varieties.
With kind permission of R. Knoblauch, LTZ Referat 23, Karlsruhe
B: SDS-PAGE ROTI®Mark Standard.
ROTI®Blue is approximately 5 x more sensitive than standard Coomassie® staining solutions. Staining time takes 2 to 12 hours depending on the desired band intensity.
Due to the fact that proteins may be stained without fixing the gel, membrane transfer and gel elution are still possible following staining.
Particularly well suited for staining prior to MS-analyses (e. g. MALDI) and prior to gel elution.
Technische informatie
| Sensitiviteit | 10 |
| Toepassing | Coomassie® staining solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
Suitability of Protein Stainings
| Product | Sensitivity | Rapid Gel Analysis |
Gel Drying |
Elution/ Transfer |
2D- Gels |
MS- Analysis |
| ROTI®Black P | 1 ng/mm2 | - | +++ | - | +++ | - |
| ROTI®Blue | 10 ng/mm2 | - | +++ | +++ | - | +++ |
| ROTI®Blue quick | 10 ng/mm2 | +++ | +++ | ++ | - | ++ |
| ROTI®Blau R | 50 ng/mm2 | +++ | + | - | - | - |
| +++: recommended +: suitable -: not recommended | ||||||
Summary Staining of Protein Gels
| Product | Sensitivity | Description |
| ROTI®Black P | 1 ng/mm2 | Silver staining kit. Highly sensitive. Simple, fast and reproducible. |
| ROTI®Blue | 10 ng/mm2 | Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
| ROTI®Blue quick | 10 ng/mm2 | Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. |
| ROTIPHORESE®Blau R | 50 ng/mm2 | Coomassie® staining solution concentrate for rapid staining. |
| Coomassie® Blue G250 (C.I. 42655) | 60 ng/mm2 | Dye for performing Coomassie® staining. ODmax = 595 |
| Coomassie® Blue R250 (C.I. 42660) | 50 ng/mm2 | Dye for performing Coomassie staining. ODmax = 550 |
| Appearance | dark blue, colloidale solution |
| pH-value | 1 - 4 |
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