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ROTI®Cell Water, 1 l

ROTI®Cell Water, 1 l

SKU 9186.2
€ 27,07
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ROTI®Cell Water, 1 l
Product Details
CAS number: 85316-98-9
Chemical formulas: C15H31NO6
HS code: 28539010
Brand: Carl Roth
ROTI®CELL Water, sterile, ready-to-use, CELLPURE®, Molar mass (M) 18,02 g/mol, Density (D) 1 g/cm³, Boiling point (bp) 100 °C, Storage temp. +2 to +25 °C, CAS No. [7732-18-5], EG-Nr. 231-791-2, Empirical formula H2O
Steriel


ROTI®Cell Water CELLPURE® ready-to-use, sterile

Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Gebruikstip Once opened, store at +2 °C to +8 °C and use within 2 years.
The buffer system in cell culture media

In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.


Sterility complies
Endotoxins <1,0 EU/ml
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