ROTI®Fect RNAi Kit, 0.2 ml

New, powerful transfection reagent for liposoma mediated siRNA and miRNA transfection of all mammalian cells in culture. Liposoma formulation and enclosed buffer were specially established and designed for core parameters of RNAi: Most efficient quantitative endocytosis plus release of nucleic acids into the cytosol, without subsequent transport to the nucleus.
ROTI^®Fect RNAi provides you with superior knockdown results, even using low amounts of RNA.Hereby, the ROTI^®Fect technology guarantees minimisation of toxic effects during transfection, thus making these reagents suitable for very sensitive cell lines of primary cells.
The detailed instruction-for-use contains a standardised transfection protocol working via a definite RNA/liposoma ratio and providing good results starting with the very first assay - making elaborate optimisations of transfection obsolete. Evaluation of results is often possible after 48 hours, enabling two sequential assays per week.

Primary cells: MCS, M1
Cell lines: A549, 3T3-L1, B16F10, CHO-K1, H441, HT1080, HeLa, HeLa-KB, HepG2

0.2 and 1 ml, respectively, ROTI^®Fect RNAi liposoma formulation (Art. No. 2911), and 0.4 and 2 ml, respectively, ROTI^®Fect RNAi buffer (Art. No. 2913). Les composants unitaires de ce kit ne peuvent pas être achetés séparément.