ROTI®Phenol/Chloroform/Isoamyl alcohol, 250 ml
SKU A156.1
€ 101,12
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I hereby confirm that:-That the substances, preparations and products are only used in an authorized manner (in accordance with European and national legislation), use these products only for research, scientific and educational purposes. The substance(s) ordered: -Not used to produce substances or to serve as a component or intermediate of narcotic drugs and/or psychotropic substances. -Not used in medicinal products for human or veterinary use, agriculture, food or cosmetics, except as a raw material, auxiliary or operating material and/or as a research and analytical reagent. - Not used to develop or produce chemical weapons - Not used for military or illegal purposes. - do not re-export the item(s) to third parties countries without prior approval of the export control authorities. -Will not be re-exported/exported or otherwise resold or transferred domestically or internationally to any destination subject to an EU, UN or US embargo, if that act would be contrary to the conditions of that embargo. - Only supplies the mentioned products to customers who can also provide such an End Use Certificate. We will carefully store all certificates received and provide them upon request. - I am over 18 years old.
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ROTI®Phenol/Chloroform/Isoamyl alcohol, 250 ml
Product Details
P-phrases: P280 P302+P352 P305+P351+P338
H-phrases: H302-H315-H319
HS code: 29071100
Brand: Carl Roth
ROTI®Phenol/Chloroform/Isoamyl alcohol, for extraction of nucleic acids, ready-to-use, Density (D) 1,2 to 1,3 g/cm³, Flash point (flp) >80 °C, Boiling point (bp) >61 °C, Storage temp. +4 °C, UN-Nr. 2810
Please note our Technical Information Brochure on the Internet next to the products:
Phenolic DNA Isolation
Background and protocol
Mechanism
Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.
- Reduce exposure to toxic chemicals
- Prepared from phenol of highest purity
- Packed under argon for maximum stability
- Successfully tried and tested in many research laboratories
ROTI®Phenol/Chloroform/Isoamyl alcohol ready-to-use, for the extraction of nucleic acids
ROTI®Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol.
ROTI®Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI®Phenol.
Mechanisme
Addition of 1 volume ROTI®Phenol/Chloroform/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.
Draw solution from lower phase. Do not shake before use!
| Bottled under argon. |
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