Verzending 24–48 u • Levering in de hele EU • Veilige chemieverpakking
ROTI®-Mark WESTERN PLUS, 500 µl, 2 x 250 µl
SKU 2245.2
€ 534,89
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ROTI®-Mark WESTERN PLUS, 500 µl, 2 x 250 µl
Product Details
HS code: 38229000
Brand: Carl Roth
ROTI®Mark WESTERN PLUS, Protein marker, prestained, ready-to-use, Density (D) ~1 g/cm³, Storage temp. -20 °C
ROTI®-Mark WESTERN PLUS ready-to-use, pre-dyed
The ROTI®Mark WESTERN PLUS protein ladder covers a wide range of molecular weights from 15 to 200 kDa. It consists of 10 IgG binding proteins with two in their mass enhanced reference bands (30 kDa and 80 kDa).
For the most common type of primary antibodies are IgG type antibodies the ROTI®Mark WESTERN PLUS ladder can be used in almost any situation of Western Blot. Appropriate detection methods are fluorescence, chemoluminescence, colour or other systems.
Gebruiksinstructies
For controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot the ladder additionally contains four prestained proteins: 10 kDa (blue), 25 kDa (green), 45 kDa (blue) und 70 kDa (red).
Fig.: Marker after detection on X-ray film (left), after Western blot on PVDF membrane (right)
Technische informatie
| Aantal fragmenten | 14 |
| Aantal proteïnebanden | 14: 10 lgG binding 4 prestained (tricolored) |
| Aantal sporen | ~200/ml |
| Kleuring | prestained |
| Groottebereik | 10-200 kDa |
| Bewijs in de westernblot | detection by anti-His possible |
| Kwantificering | not recommended |
| Verzending | ready-to-use |
| Versterkte bande bij | 30 kD, 80 kD |
Prestained Protein Markers
| Marker | ALL BLUE | BI-PINK | TRICOLOR | TRICOLOR XTRA |
| Art. No. | 2242 (fig. 1) | 8269 (fig. 2) | 8271 (fig. 3) | 2244 (fig. 4) |
| Size Range (kD) | 10-240 | 10-175 | 10-245 | 10-310 |
| Number of Fragments | 12 | 11 | 12 | 12 |
| Reinforced band at (kD) | 25, 72 | - | - | - |
| Quantitation | not recomm. | not recomm. | not recomm. | not recomm. |
| Staining | blue prestained | pink prestained | blue prestained | blue prestained |
| Additional reference bands | - | 10 kD, 40 kD, 90 kD blue | 25 kD green, 75 kD red | 25 kD green, 75 and 310 kD red |
| Detection in Western-Blot | no detection | no detection | no detection | no detection |
| Number of Lanes in minigel | 200 / ml | 200 / ml | 200 / ml | 200 / ml |
| Delivery | ready-to-use | ready-to-use | ready-to-use | ready-to-use |
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