Sterile ready-to-use culture media plates for microbiological analysis.
ROTI®Plate90 are prepared culture media plates with a diameter of 90 mm. They are thus adapted to the size of membrane filters for assaying of low-germ fluids by membrane filtration method. They are also applicable for the microbiological analysis of samples in general.
The germs of expected low-germ liquid samples are concentrated by membrane filtration. Afterwards, the membrane is placed on top of the medium and incubated. Using a membrane with a grid helps counting the resulting colonies.
Our products are tested and approved exclusively for research and development.
Samples which are expected to have a higher microbiological burden are prepared in a serial dilution. Afterwards, every dilution step is plated and incubated. The number of grown colonies is noted as result.
Pseudomonas CN Agar is a selective medium for the isolation and enumeration of Pseudomonas aeruginosa by membrane filtration.
It is recommended by ISO Standard 16266:2008 for testing water samples for the presence of Pseudomonas aeruginosa.
Cetrimide is included in the formulation of the medium as a selective agent, nalidixic acid suppresses species such as Klebsiella, Proteus and Providencia. Potassium sulphate and magnesium chloride provide the cations needed to activate pyocyanin production.
Toepassing | Pseudomonas aeruginosa |
Gelatin Peptone | 16 g/l |
Casein, enzymatically digested | 10 g/l |
Potassium sulphate (K2SO4) | 10 g/l |
Glycerol | 10 ml |
MgCl2 | 1.4 g/l |
Cetrimide | 0.2 g/l |
Nalidixic acid | 0.015 g/l |
Agar-Agar | 13 g/l |
pH value (25 °C) | 6.9-7.3 |