Sterile ready-to-use culture media plates for microbiological analysis.
ROTI®Plate90 are prepared culture media plates with a diameter of 90 mm. They are thus adapted to the size of membrane filters for assaying of low-germ fluids by membrane filtration method. They are also applicable for the microbiological analysis of samples in general.
The germs of expected low-germ liquid samples are concentrated by membrane filtration. Afterwards, the membrane is placed on top of the medium and incubated. Using a membrane with a grid helps counting the resulting colonies.
Our products are tested and approved exclusively for research and development.
Samples which are expected to have a higher microbiological burden are prepared in a serial dilution. Afterwards, every dilution step is plated and incubated. The number of grown colonies is noted as result.
Selective medium for the detection and quantitative determination of Enterococcus spp.
The TTC contained in the medium is reduced by Enterococcus to the water-insoluble dye formazan. The resulting formazan is taken up by Enterococcus spp., which gives the colonies a red to dark brown colour.
Toepassing | Enterococcus spp. |
Casein, tryptic digest | 20 g/l |
Yeast extract | 5 g/l |
Glucose | 2 g/l |
Dikaliumhydrogenphosphat | 4 g/l |
Sodium azide | 0.4 g/l |
Tetrazolium chloride (TTC) | 0.1 g/l |
Agar-Agar | 10 g/l |
pH value (25 °C) | 7.1-7.3 |