Sterile ready-to-use culture media plates for microbiological analysis.
ROTI®Plate90 are prepared culture media plates with a diameter of 90 mm. They are thus adapted to the size of membrane filters for assaying of low-germ fluids by membrane filtration method. They are also applicable for the microbiological analysis of samples in general.
The germs of expected low-germ liquid samples are concentrated by membrane filtration. Afterwards, the membrane is placed on top of the medium and incubated. Using a membrane with a grid helps counting the resulting colonies.
Our products are tested and approved exclusively for research and development.
Samples which are expected to have a higher microbiological burden are prepared in a serial dilution. Afterwards, every dilution step is plated and incubated. The number of grown colonies is noted as result.
Endo Agar is used for the differentiation of lactose-fermenting and non-lactose-fermenting intestinal bacteria, specifically to confirm tests when coliforms are suspected.
The production of acid and aldehyde by lactose fermenting organisms such as Escherichia coli, results in the characteristic red colouration of the colony and the surrounding medium, along with a brilliant golden metallic sheen.
Non-lactose fermenting bacteria result in colourless and transparent colonies.
Meat peptone | 10 g/l |
Lactose | 10 g/l |
Dikaliumhydrogenphosphat | 3.5 g/l |
Sodium sulphite | 2.5 g/l |
Fuchsine basic | 0.5 g/l |
Agar-Agar | 15 g/l |
pH value (25 °C) | 7.2-7.6 |