Sterile ready-to-use culture media plates for microbiological analysis.
ROTI®Plate90 are prepared culture media plates with a diameter of 90 mm. They are thus adapted to the size of membrane filters for assaying of low-germ fluids by membrane filtration method. They are also applicable for the microbiological analysis of samples in general.
The germs of expected low-germ liquid samples are concentrated by membrane filtration. Afterwards, the membrane is placed on top of the medium and incubated. Using a membrane with a grid helps counting the resulting colonies.
Our products are tested and approved exclusively for research and development.
Samples which are expected to have a higher microbiological burden are prepared in a serial dilution. Afterwards, every dilution step is plated and incubated. The number of grown colonies is noted as result.
MRS Agar was developed by deMan, Rogosa and Sharpe to grow luxuriantly all strains of Lactobacilli especially the fastidious, slower-growing types such as L. brevis and L. fermenti. The medium is apt for the growth of lactic acid bacteria, including Lactobacillus, Streptococcus, Pediococcus and Leuconostoc.
Ammonium citrate at a low pH inhibits most microorganisms, including other Streptococci and molds. It allows for the growth of Lactobacillus, but limits swarming in general.
Casein, enzymatically digested | 10 g/l |
Glucose | 20 g/l |
Yeast extract | 4 g/l |
Beef extract | 10 g/l |
Dikaliumhydrogenphosphat | 2 g/l |
Magnesium sulphate (MgSO4) | 0.2 g/l |
Sodium acetate | 5 g/l |
Manganese sulphate | 0.05 g/l |
Triammonium citrate | 2 g/l |
Tween 80 | 1 g/l |
Agar-Agar | 10 g/l |
pH value (25 °C) | 6.0-6.4 |