Sterile ready-to-use culture media plates for microbiological analysis.
ROTI®Plate90 are prepared culture media plates with a diameter of 90 mm. They are thus adapted to the size of membrane filters for assaying of low-germ fluids by membrane filtration method. They are also applicable for the microbiological analysis of samples in general.
The germs of expected low-germ liquid samples are concentrated by membrane filtration. Afterwards, the membrane is placed on top of the medium and incubated. Using a membrane with a grid helps counting the resulting colonies.
Our products are tested and approved exclusively for research and development.
Samples which are expected to have a higher microbiological burden are prepared in a serial dilution. Afterwards, every dilution step is plated and incubated. The number of grown colonies is noted as result.
MacConkey agar is used for the subcultivation of Gram-negative enterobacterial colonies and for the determination of Escherichia coli.
Bile salts and crystal violet are the selective agents and inhibit Gram-positive bacteria.
Neutral red is the pH indicator that, when lactose is fermented and the pH drops, shows a colour change from neutral red to pink.
Casein peptone | 17 g/l |
Lactose | 10 g/l |
Sodium chloride (NaCl) | 5 g/l |
Meat peptone | 3 g/l |
Bile salts No. 3 | 1.5 g/l |
Neutral red | 0.03 g/l |
Crystal Violet | 0.001 g/l |
Agar-Agar | 13.5 g/l |
pH value (25 °C) | 6.9-7.3 |