ROTI®Pol Hot-TaqUltra, 40 µl, 1 x 40 µl

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI^®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI^®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

Hot start version of the recombinant heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer and tested on the absence of bacterial DNA. ROTI^®Pol Hot-TaqUltra is recommended for use in highly specific PCR applications in general, or if bacterial DNA, or in RT-PCR, bacterial 16S rRNA shall be detected.
ROTI^®Pol Hot-TaqUltra is purified using a multiple-step process that minimizes contaminating bacterial DNA to a none detectable level. Each lot of the polymerase undergoes strict quality control testing in order to ensure the absence of detectable amounts of contaminating bacterial DNA. ROTI^®Pol Hot-TaqUltra is provided in a DNA-free storage buffer containing 50 % glycerol.
ROTI^®Pol Hot-TaqUltra is recommended for use when particularly specific amplification of bacterial DNA is the main focus. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.
ROTI^®Pol Hot-TaqUltra is able to amplify PCR products up to 5 kb and is appropriate for use in the amplification of DNA from eukaryotic as well as prokaryotic templates. The Hot-TaqUltra DNA polymerase possesses a 5’ → 3’ polymerase- as well as a 5’-3' exonuclease activity, and generates a 3'dA (adenine)-overhang which may well be used for TA-cloning purposes.