ROTI®Pol TaqHY Mix (2x), 10 ml, 10 x 1 ml

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI^®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI^®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

Optimized, pre-mixed 2x PCR solution containing the recombinant TaqHY DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl₂ and all other components required for PCR except primers and template DNA. ROTI^®Pol TaqHY Mix (2x) is recommended for all PCR applications where very short cycles or high yield are required.

PCR assaying with ROTI^®Pol TaqHY Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI^®Pol TaqHY Mix (2x) shows both an enhanced polymerase activity and an extremely time-saving, fast elongation rate.

Due to the optimized composition of the master mix, the TaqHY polymerase offers specific PCR amplification with good yield on a wide range of PCR templates. The yield achieved is significantly higher than that of conventional Taq polymerases, with shorter cycle times. ROTI^®Pol TaqHY Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. The use of the colourless PCR Master mix is particularly recommended when direct fluorescence or absorbance readings are required. The TaqHY DNA polymerase included in the mastermix possesses a 5' →3' polymerase as well as a 5'-flap endonuclease activity and generates a 3'dA (adenine) overhang which can be used for TA cloning purposes.

2 or 10 vials, respectively, with 1 ml each of ROTI^®Pol TaqHY Mix (2x) containing TaqHY polymerase, 0.4 mM each dNTP, and 4 mM MgCl₂ in 2x reaction buffer