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ROTI®Pol TaqS Mix (2x), 2 ml, 2 x 1 ml
SKU 9239.1
€ 59,70
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ROTI®Pol TaqS Mix (2x), 2 ml, 2 x 1 ml
Product Details
HS code: 38229000
Brand: Carl Roth
ROTI®Pol TaqS Mix (2x), ready-to-use, 2x conc., Density (D) ~1 g/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C
For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.
ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.
ROTI®Pol TaqS Mix (2x) 2x conc., ready-to-use
Optimized pre-mixed 2x PCR solution containing the recombinant TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl2, and all other components required for PCR except primers and template DNA. ROTI®Pol TaqS Mix (2x) is recommended for use in all standard PCR applications.
PCR assaying with ROTI®Pol TaqS Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI®Pol TaqS Mix (2x) can be applied to all standard Taq-based cycling protocols and is, therefore, the optimal choice for high throughput PCR as being performed in, for instance, analysis of cloning efficiency or colony screening, for routine screening processes, and for student's courses.
Due to the optimized composition of the master mix, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates. ROTI®Pol TaqS Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. The use of the colourless PCR Master mix is particularly recommended when direct fluorescence or absorbance readings are required. The TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.
Figure: Sensitivity assay using ROTI®Pol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.
M: 100 bp-DNA Ladder extended. NTC: no template control.
M: 100 bp-DNA Ladder extended. NTC: no template control.
De set bevat
Composition:
2 or 10 vials, respectively, with 1,25 ml each of ROTI®Pol TaqS Mix (2x) containing TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl2 in 2x reaction buffer
Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
| Toepassing | Standard PCR |
| Amplicon-einden | 3'dA |
| Master-mix | colourless |
| Polymerase | Standard Taq polymerase |
| Toepassing | Standard PCR |
| Appearance | colourless |
| Endonuclease activity | none detected |
| Exonuclease activity | none detected |
| Suitability for PCR (400 bp) | complies |
| Suitability for PCR (3 kb) | complies |
| Selective specificity in PCR | complies |
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