ROTI®Prep Genomic DNA MINI, 250 preparations
SKU 8472.3
€ 787,95
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I hereby confirm that:-That the substances, preparations and products are only used in an authorized manner (in accordance with European and national legislation), use these products only for research, scientific and educational purposes. The substance(s) ordered: -Not used to produce substances or to serve as a component or intermediate of narcotic drugs and/or psychotropic substances. -Not used in medicinal products for human or veterinary use, agriculture, food or cosmetics, except as a raw material, auxiliary or operating material and/or as a research and analytical reagent. - Not used to develop or produce chemical weapons - Not used for military or illegal purposes. - do not re-export the item(s) to third parties countries without prior approval of the export control authorities. -Will not be re-exported/exported or otherwise resold or transferred domestically or internationally to any destination subject to an EU, UN or US embargo, if that act would be contrary to the conditions of that embargo. - Only supplies the mentioned products to customers who can also provide such an End Use Certificate. We will carefully store all certificates received and provide them upon request. - I am over 18 years old.
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ROTI®Prep Genomic DNA MINI, 250 preparations
Product Details
P-phrases: P201 P280 P301+P310 P302+P352 P305+P351+P338 P308+P313
H-phrases: H301-H315-H317-H319-H332-H340-H350-H361f-H372
Hazard class: 6.1 III
HS code: 38229000
Brand: Carl Roth
ROTI®Prep Genomic DNA MINI, for molecular biology, Flash point (flp) 21 °C, Boiling point (bp) 82 °C, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep Genomic DNA MINI for molecular biology
Roti®Prep Genomic DNA MINI has been developed for the isolation of genomic DNA from various sources.
The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps.
- Preparation by well-known mini spin-column system
- Fast, easy and efficient
- Preparation in approx. 15 minutes (excluding lysis)
- Yield up to 65 μg gDNA, depending on source material
In addition to the especially on proteinase K adapted lysis buffer, the filtration spin column has been optimised, permitting particularly high extraction rates. Extraction from up to 40 mg tissue, therefore, allows a yield of up to μg µg DNA. The thereby extracted DNA is free of RNA and proteins, and can directly be stored or used for downstream applications.
Mogelijk uitgangsmateriaal:
- Tissue (up to 40 mg)
- Rodent tails (0,5-1 cm)
- FFPE tissue (formalin-fixed, paraffin-embedded)
- Buccal swabs
- Cell culture (up to 5x106 cells)
Figure:
Typical test gel following isolation of genomic DNA (A) and subsequent GAPDH-PCR (B) from rodent tail (frozen, 8 mm each).
Yield of gDNA (from left to right): 22,6 μg, 27,7 μg and 28 μg. Ratio A260:A280 = 1,97±0,01.
Typical test gel following isolation of genomic DNA (A) and subsequent GAPDH-PCR (B) from rodent tail (frozen, 8 mm each).
Yield of gDNA (from left to right): 22,6 μg, 27,7 μg and 28 μg. Ratio A260:A280 = 1,97±0,01.
The kit contains:
Lysis Buffer LSA, Binding Buffer BSN, Proteinase K, Washing Buffer WST, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use.
Contents of this kit may not be bought separately.
Please order ethanol 99,8 % (p.a., Art. No. 9065), RNase A (Art. No. 7156, not for FFPE tissue), and for FFPE tissue xylole (Art. No. 4436) or octane separately.
Geen medisch hulpmiddel / Geen ivD-product
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