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ROTI®Quick kit

ROTI®Quick kit

SKU A979.1
€ 120,80
In stock
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ROTI®Quick kit
Product Details
P-phrases: P210 P270 P280 P302+P352 P304+P340 P308+P311
H-phrases: H225-H301+H311+H331-H370 EUH208
Hazard class: 3 (6.1) II
HS code: 38229000
Brand: Carl Roth
ROTI®Quick kit, ready-to-use, for molecular biology, Density (D) 0,786 g/cm³, Storage temp. +4 °C, UN-Nr. 2924


ROTI®Quick kit ready-to-use, for molecular biology

Column-free, ultra-flexible isolation system designed for reliable isolation of total RNA from almost any tissue.
The ROTI®Quick Kit is suitable for use with cell culture material, biopsies, high-fat or high-protein tissue, blood (Üçeyler et al. Neurology (2007) 69:42-9), solid plant material, mitochondria (Backert et al. Plant Molecular Biology (1997) 33:1037-50), mycoplasms (Weiner et al. Nucleic Acids Res. (2000) 28:4488-96) and many more. The kit can be used for both fresh tissue and frozen material.

The ROTI®Quick Kit is based on the GITC isolation method by Chomczynski and Sacchi (Chomczynski and Sacchi. (1987) Anal. Biochem. 162:156) and includes three optimised ready-to-use solutions that were developed by molecular biologists and have proven effective for many years.
After the tissue has been homogenised, the RNA is purified in a single step and isolated by precipitation. Each isolation takes approximately 2,5 hours, and multiple isolations can easily be performed simultaneously. The incubation and centrifuging steps take about 2 hours.

  • Easy, most versatile application
  • High recovery rate of intact, very pure total RNA
  • Column-free two-step isolation
  • Applicable on nearly any tissue type

Preparation as given in the instruction-for-use is optimised for RNA isolation from approx. 0,2 g of tissue or cell material. The amount of material isolated per batch may easily be up- or downscaled to larger or smaller amounts of tissue.
The isolated total RNA is highly pure and may be used directly for all common down-stream applications, such as RT-PCR, Northern blotting and reverse transcription. Detailed instructions-for-use are enclosed with each kit.

A979_Ablaufschema

The kit contains:

ROTI®Quick Kit 1, ROTI®Quick Kit 2 and ROTI®Quick Kit 3.
Contents of this Kit may not be bought separately.



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