10fold stock solution tris buffered saline, pH 7,4.
Very versatile standard solution for detection systems in protein biochemistry, cytological stainings and molecular biological assays.
1 x TBS is, for instance, used as basic solution for protein detection systems like Western-Blot analyses and Far-Western assays, for immunocytological and immunohistological assays, as well as for whole mount in situ hybridisations, apoptosis detection systems and staining of nuclei.
pHwaarde | 7,4 ±0,25 |
Appearance of solution | colourless liquid |
Colour | water-clear |
pH value | 7.15 - 7.65 |
1.5 M NaCl, 30 mM KCI, 250 mM Tris in deionised water, pH value 7.4 ±0.25, 0.2 μm filtered and steam sterilised. |