The TBE buffer is optimally suited as a gel and running buffer system.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.
ROTIPHORESE®10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3.
Suitable for manufacturing sequencing gels with ROTIPHORESE®Gel 40 and as a running buffer.
ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
1.0 M TRIS borate (pH 8.3), 20 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C). |