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ROTIPHORESE®10x TBE Buffer, 5 l, plastic, 1 x 5 l

ROTIPHORESE®10x TBE Buffer, 5 l, plastic, 1 x 5 l

SKU 3061.2
€ 128,29
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ROTIPHORESE®10x TBE Buffer, 5 l, plastic, 1 x 5 l
Product Details
P-phrases: P280 P302+P352 P305+P351+P338
H-phrases: H302-H315-H319-H335
CAS number: 96736-11-7
HS code: 38229000
Brand: Carl Roth
ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, Density (D) 1,068 g/cm³, Boiling point (bp) >100 °C, Storage temp. +15 to +25 °C

The TBE buffer is optimally suited as a gel and running buffer system.


Mechanisme

TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.



ROTIPHORESE®10x TBE Buffer ROTIPHORESE® 10x conc.

ROTIPHORESE®10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3.

Suitable for manufacturing sequencing gels with ROTIPHORESE®Gel 40 and as a running buffer.


Gebruiksinstructies

ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.



1.0 M TRIS borate (pH 8.3), 20 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).
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