SYBR® Green DNA dye, 9.0 ml, 5 x 1.8 ml

This is the DNA dye SYBR^® Green I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/loading buffer mixture and then loaded onto the gel.
SYBR^® Green I bounds to the minor groove of DNA and intercalates into the DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR^® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.

SYBR^® Green DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE^®PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or SYBR^® Green foto filters.

Excitation maximum (bound to DNA): approx. 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm