Home/Store/Laboratory supplies/Filtration, water treatment, dialysis/Filtration and separation/Membrane filters
Transfer membrane Amersham Protran® NC 0.45 roll 400 × 30 cm, 1 Piece(s)

Transfer membrane Amersham Protran® NC 0.45 roll 400 × 30 cm, 1 Piece(s)

SKU 4675.1
€ 697,05
In stock
1
Save this product for later
Transfer membrane Amersham Protran® NC 0.45 roll 400 × 30 cm, 1 Piece(s)
Product Details
HS code: 39207990
EAN: 8721028170262
Brand: Cytiva
Material: nitrocellulose (100 %).

Transfer membrane for most nucleic acid and protein immobilising techniques

Replaces Protran® BA 85 from Whatman®

Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background.


Directions for use

Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer.
Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).


Application examples

Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.
Detection systems are radioactivity, chemoluminescence, colour



Transfer membrane Amersham™ Protran® NC 0.45 roll 400 × 30 cm

In practical roll format for maximum flexibility in cutting.



Technical Information
Dimensions 400 × 30 cm
Version roll
Pore size 0,45 µm
Type Protran® NC 0,45
Bond electrostatic, hydrophobic
Binding capacity 86-110 µg/cm²
Immobilisation UV, baking (max. 80 °C)

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Show More
You May Also Like
3-hydroxy-3,3-diphenylpropanehydrazide, 1g
3-hydroxy-3,3-diphenylpropanehydrazide, 1g
3-hydroxy-3,3-diphenylpropanehydrazide, 1g
SKU F510702-1G
€ 363,00
3-METHOXY-1,6-NAPHTHYRIDIN-5(6H)-ONE, 95.0%, 100mg
3-METHOXY-1,6-NAPHTHYRIDIN-5(6H)-ONE, 95.0%, 100mg
3-METHOXY-1,6-NAPHTHYRIDIN-5(6H)-ONE, 95.0%, 100mg
SKU F503417-100MG
€ 323,40
3-methylimidazo[1,2-a]pyridine-2-carboxylic acid, 95.0%, 5g
3-methylimidazo[1,2-a]pyridine-2-carboxylic acid, 95.0%, 5g
3-methylimidazo[1,2-a]pyridine-2-carboxylic acid, 95.0%, 5g
SKU F401744-5G
€ 952,60
Boc-2-aminobenzoic acid, 95.0%, 100g
Boc-2-aminobenzoic acid, 95.0%, 100g
Boc-2-aminobenzoic acid, 95.0%, 100g
SKU F237131-100G
€ 484,00
(4-Methoxy-benzyl)-piperidin-4-yl-amine hydrochloride, 95.0%, 100mg
(4-Methoxy-benzyl)-piperidin-4-yl-amine hydrochloride, 95.0%, 100mg
(4-Methoxy-benzyl)-piperidin-4-yl-amine hydrochloride, 95.0%, 100mg
SKU F089113-100MG
€ 111,10
2-Fluoro-5-nitrobenzoic acid, 98.0%, 10g
2-Fluoro-5-nitrobenzoic acid, 98.0%, 10g
2-Fluoro-5-nitrobenzoic acid, 98.0%, 10g
SKU F009222-10G
€ 17,60
1-Butylthiourea>98.0%(N)5g
1-Butylthiourea>98.0%(N)5g
1-Butylthiourea>98.0%(N)5g
SKU B4971-5G
€ 273,90
Methyl 5-methylpyrazolo[1,5-a]pyridine-3-carboxylate, 97%, 100mg
Methyl 5-methylpyrazolo[1,5-a]pyridine-3-carboxylate, 97%, 100mg
Methyl 5-methylpyrazolo[1,5-a]pyridine-3-carboxylate, 97%, 100mg
SKU F612123-100MG
€ 55,00
2,3-Difluoro-5-methylphenylboronic acid, 98%, 5g
2,3-Difluoro-5-methylphenylboronic acid, 98%, 5g
2,3-Difluoro-5-methylphenylboronic acid, 98%, 5g
SKU F623013-5G
€ 193,60
Potassium phosphate dibasic, 98.0%, 500g
Potassium phosphate dibasic, 98.0%, 500g
Potassium phosphate dibasic, 98.0%, 500g
SKU F094672-500G
€ 22,00
pentanedihydrazide, 95.0%, 5g
pentanedihydrazide, 95.0%, 5g
pentanedihydrazide, 95.0%, 5g
SKU F369131-5G
€ 303,60
7-Chloroquinaldine, 99.0%, 1g
7-Chloroquinaldine, 99.0%, 1g
7-Chloroquinaldine, 99.0%, 1g
SKU F002536-1G
€ 18,70
  • Search Products
  • My Account
  • Track Orders
  • Favorites
  • Shopping Bag
Powered by Lightspeed
Display prices in:EUR
Doorgaan naar hoofdinhoud
SciSupplies
NL
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Menu
Winkel
Over
Neem contact met ons op
FAQ
Blog

Onderdeel van HD Beverwijk Trading

Taal:
NL
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Algemene voorwaardenPrivacybeleidVerzend- en betaalinformatieRetourbeleidRapporteer misbruik
Powered by Lightspeed