Transfer membrane ROTI®FluoroPVDF, 20 × 13 cm, 2 Piece(s)

PVDF membrane for protein analyses.

ROTI^®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.

The membrane shows superior protein binding parameters and stands out for its particularly low background. It may also be used very well after repeated stripping and harsh washing processes.
This mechanically rigid transfer membrane can be easily cut to the desired gel size and it remains flexible and tear-proof even when dry.
Briefly immerse the membrane membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every blotting system.
Due to production processes, the membrane shows two different sides - one smooth and one raw. For optimally distinct bands, please use the smooth side for protein binding.

ROTI^®Fluoro PVDF has been specially optimised for direct detection via fluorescence.
ROTI^®Fluoro PVDF binds proteins adhesively via hydrophobic interactions. It enables very sensitive protein detection by keeping the background very low, resulting in a good signal-to-noise ratio with all standard detection systems. The mechanically rigid membrane is of high tensile strength and tear-proof, and does not crack or curl during handling. Therefore, signals remain clear and distinct, and are undisturbed by false-positive signals caused by membranes crinkles.
Briefly immerse ROTI^®Fluoro PVDF membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every aqueous transfer system.

The membrane ROTI^®Fluoro PVDF is only conditionally suitable for use with chemiluminescence detection. The membranes are specially coated for fluorescence applications and this coating can reduce the sensitivity of chemiluminescence. For chemiluminescent assays we recommend the use of ROTI^®PVDF membranes.
Detection systems: fluorescence, radioactivity, colour