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Transfer membrane ROTI®NC 0.45, 20 × 20 cm, 5 Piece(s)

Transfer membrane ROTI®NC 0.45, 20 × 20 cm, 5 Piece(s)

SKU 200N.1
€ 126,53
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Transfer membrane ROTI®NC 0.45, 20 × 20 cm, 5 Piece(s)
Product Details
HS code: 48232000
EAN: 8721028170200
Brand: Ratiolab GmbH
Material: nitrocellulose (100 %).

Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.



Transfer membrane ROTI®NC 0.45

The pore size of 0,45 μm is suitable for proteins and peptides of all sizes. The best retention rate is achieved when blotting proteins of ≥20 kDa. Having a high protein binding capacity, the membrane not only efficiently prevents protein loss during transfer, but also guarantees very sensitive detection of even low protein amounts.

A slight optical difference between the two membrane sides results from the membrane production and has no influence on the binding capacity or the blot quality of the two sides. Both sides can be used equally for transfer and detection.We recommend to always use the optically same side as “detection side” to achieve maximum reproducibility.

Optimised for proteins ≥20 kDa. Western- and far-Western-blots, Southern- and Northern blots, colony- and plaque-lifting of nucleic acids and proteins, dot- and slot blots.
Detection via radio activity, chemoluminescence, colour.

Suitable for:
  • Western blotting
  • Protein and immunoblotting
  • Northern blotting
  • Southern blotting
  • Dot / slot blotting


Not a medical device / Not an IVD product
Technical Information
Dimensions 20 × 20 cm
Version roll
Pore size 0,45 µm
Bond hydrophobic
Binding capacity 100 µg/cm²
Thickn. 150±50 µm
Immobilisation UV, baking (max. 80 °C)

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

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