Transfer membrane ROTI®Nylon plus, 300 × 30 cm, 1 Piece(s)
SKU K058.1
€ 362,73
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Transfer membrane ROTI®Nylon plus, 300 × 30 cm, 1 Piece(s)
Product Details
HS code: 39209200
Material: Nylon
EAN: 8721028171481
Brand: Carl Roth
Material: nylon 6.6.
.
Transfer membrane ROTI®Nylon plus
Ideal for DNA/RNA-transfers.
ROTI®Nylon plus is a cationic, einforced nylon 6.6-membrane with extremely high tensile strength. The positively charged membrane surface guarantees rapid and efficient binding of DNA and RNA. May be steam sterilised.
Also available in convenient pre-cut sheets of standard gel size!
Nucleic acids can be cross-linked to the membrane by baking (80 °C, 15 min) or through UV exposure. No cross-linking is required for alkaline transfer as the negatively charged DNA forms a very stable complex with the positively charged ROTI®Nylon membrane.
Detection systems: radioactivity, chemoluminescence, fluorescence
Application examples:
- Southern and Northern blot
- Dot-Blot
- DNA fngerprinting
- Macro- and Microarrays
Technical Information
| Dimensions | 300 × 30 cm |
| Version | roll |
| Pore size | 0.45 µm |
| Type | 0.45 µm |
| Bond | hydrophilique |
| Load | positive |
| Binding capacity | 500 µg/cm² |
| Thickn. | 160±20 µm |
| Immobilisation | not necessary, UV and baking possible |
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Roti® PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 140 ±20 | ≥125 | 0,2 | not necessary |
| Roti® PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | ca. 200 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Roti® NC | Nitrocellulose | electrostatic, hydrophobic | 150 ±50 | 125-200 | 0,2 | UV, baking (max. 80 °C) |
| Roti® NC 0.45 | Nitrocellulose | electrostatic, hydrophobic | 150 ±10 | ≥125 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
Size Comparison of Membrane Pores and Biomolecules
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
Application of ROTH-Transfer Membranes
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||
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