Trypsin inhibitor, 1 g

Trypsin inhibitor inhibits Trypsin with high effiency, and, to a lesser extent, chymotrypsin. Additionally, there is a light inhibiting effect on plasmin, kallikrein, thrombin and a few other proteolytic enzymes.

Recommended final concentration: 1-100 µg/ml

Solutions of higher concentration may be hazy and slightly yellow-coloured.
pH optimum: pH 7.6-8.0.

1 mg of lyophilisate inhibits at minimum 10.000 BAEE Units of trypsin.

Application in cell culture (stock solution 1 mg/ml): Following trypsination, add the same volume inhibitor solution as was used for trypsin. Zentrifuge cells (5 mins, 1000 rpm), and remove supernatant. Resuspend cells in medium and cultivate further.