Escherichia transform lactose under acid production via β-galactosidase, and are negative in oxidase test and positive in indole production test. Kovac Reagent (Art. No. 2950.1) is added to 24-48 h old cultures in a concentration of 0.2 to 0.5 ml per 5 ml broth. A colour shift to dark red shows presence of indole.
Gecertificeerd conform | DEV ISO 16654 |
Casein, tryptic digest | 10 g/l |
L-Tryptophan | 1 g/l |
Sodium chloride (NaCl) | 5 g/l |
pH value | 7,5 ±0,1 |