anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, 0.2 ml

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type.

Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas.

Polypeptide reacting: Mr 165 000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide.

Reactivity on cultured cell lines: A-431, MDBK.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: DG 3.10
Immunogen: 'band 3' polypeptide of isolated desmosomes from bovine muzzle epidermis
UniprotID: Q02413 (human), D3ZM39 (rat), Q03763 (bovine)
Synonym: desmoglein-1, cadherin family member 4, desmosomal glycoprotein 1, DG1, DGI, pemphigus foliaceus antigen, DSG1, CDHF4
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, human, rat

Immunocytochemistry (ICC): Assay dependent
Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, microwave treatment recommended)
Western Blot (WB): 1:2000-1:4000 (12,5 ng/ml - 25 ng/ml)