Name: anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, 0.2 ml
Brand: PROGEN
SKU: 1NP4.1
Price: 160.09 EUR
Availability: InStock

anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, 0.2 ml

Product Details

HS code: 30021500

Brand: PROGEN

anti-Perilipin 2 (N-terminus) mouse, monoclonal, AP125, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125 50 μg/ml, monoclonal, affinity purified

Perilipin 2/Adipophilin/ADRP/PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets.

Polypeptide reacting: Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.

Immunolocalization: Perilipin 2/Adipophilin/ADRP/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages.

Tested cultured cell lines: PLC, MDCK.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: AP125
Immunogen: synthetic peptide (aa 5-27 from N-terminus of human adipophilin/PLIN2)
UniprotID: A0A5F4CRI5 (dog, canis familiaris), Q99541 (human), Q5U2U5 (rat)
Synonym: perilipin-2, adipophilin, adipose differentiation-related protein, ADRP, PLIN2, ADFP
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: dog, human, rat
No reactivity: bovine

Toepassing

Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0,5-5 μg/ml; microwave treatment recommended)
Western Blot (WB): 1:50-1:100 (0.5-1 μg/ml)

Western blot analysis of HepG2 cell lysate with anti-Perilipin 2 antibody. Western blot analysis was performed on 15 μg HepG2 lysate and 15 μg HepG2 + OA lysate. Cells were previously treated with 400 μM oleic acid (OA) if indicated. Cells were lysed in RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125 (Cat. No. 690102) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated at 4°C over-night. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)

IHC of rat stomach (courtesy of J. Heß, University Hospital Heidelberg)

Geen medisch hulpmiddel / Geen ivD-product