anti-Plakoglobin mouse monoclonal, PG 5.1, 0.2 ml
SKU 1NNC.1
€ 110,31
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anti-Plakoglobin mouse monoclonal, PG 5.1, 0.2 ml
Product Details
P-phrases: P260 P270 P280 P302+P352 P305+P351+P338 P310
H-phrases: H302+H312+H332-H314-H412 EUH032
CAS number: 593-84-0
Chemical formulas: C2H6N4S
Hazard class: 8 II
HS code: 30021500
Brand: PROGEN
anti-Plakoglobin mouse, monoclonal PG 5.1, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Plakoglobin mouse monoclonal, PG 5.1 50 μg/ml, monoclonal, affinity purified
PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: desmosomes of epithelial and myocardial cells (incl. cultured cells); zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin.
Polypeptide reacting: Mr 83,000 Plakoglobin, 'band 5' polypeptide of intercellular adhering junctions (identical to gamma-catenin).
Reactivity on cultured cell lines: cell cultures forming monolayers.
Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: PG 5.1
Immunogen: plakoglobin, 'band 5' protein from bovine snout epidermis
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, dog, human, mouse, rat, zebrafish
Toepassing
Immunocytochemistry (ICC): 1:5 (10 μg/ml)
Immunohistochemistry (IHC) - frozen: 1:25-1:100 (0.5-2 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:25-1:100 (0.5-2 μg/ml, microwave treatment recommended)
Western Blot (WB): 1:500-1:2,000 (0.025-0.1 μg/ml)
IHC of human oral mucosa (courtesy of J.Heß, University Hospital Heidelberg)
IHC of rat tongue (courtesy of J.Heß, University Hospital Heidelberg)
Western blot analysis of Ins-1 lysate and with anti-Plakoglobin antibody. Western blot analysis was performed on 20 μg rat Ins-1 lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Plakoglobin mouse monoclonal, PG 5.1 (Cat. No. 690005) was diluted in blocking buffer (antibody concentration 25 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
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