Name: anti-Uroplakin III mouse monoclonal, AU1, 0.2 ml
Brand: PROGEN
SKU: 1NLK.1
Price: 110.31 EUR
Availability: InStock

anti-Uroplakin III mouse monoclonal, AU1, 0.2 ml

Product Details

CAS number: 56-45-1

Chemical formulas: C3H7NO3

HS code: 30021500

Brand: PROGEN

anti-Uroplakin III mouse , monoclonal, AU1, 100 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-Uroplakin III mouse monoclonal, AU1 100 μg/ml, monoclonal, affinity purified

Mab AU1 reacts specifically with uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kDa) and has been shown to be a specific marker of terminal urothelial differentia-tion (Wu et al. 1993 & 1194).

Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al. 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.

Moll, R., Wu, X.-R., Lin, J.-H. & Sunt, T.-T. Uroplakins, Specific Membrane Proteins of Urothelial Umbrella Cells, as Histological Markers of Metastatic Transitional Cell Carcinomas. Am. J. Pathol. 147, 1383-1397 (1995). Wu, X.-R. et al. Mammalian Uroplakins: A group of highly conserved urothelial differentiation-related membrane proteins. J. Biol. Chem. 269, 13716-13724 (1994). Wu, X. R. & Sun, T. T. Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein. J. Cell Sci. 106, 31-43 (1993).

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: AU1
Immunogen: asymmetric unit membrane (AUM) preparation from bovine urinary bladder
UniprotID: P38574 (Bovine), O75631 (Human), A0A4X1W3H8 (Pig), D3ZZ76 (Rat)
Synonym: uroplakin-3a, UP3a, uroplakin III, UPIII, UPK3, UPK3A
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: bovine, human, pig, rat

Toepassing

Immunohistochemistry (IHC) - paraffin: 1:20-1:60 (1.6-5 μg/ml; microwave treatment recommended)
Western Blot (WB): 1:4,000-1:20,000 (0.005-0.025 μg/ml)

IHC of rat bladder (courtesy of J.Heß, University Hospital Heidelberg)

Western blot analysis of human bladder whole tissue lysate with anti-Uroplakin III antibody. Western blot analysis was performed on 3 μg and 2 μg bladder lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Uroplakin III mouse monoclonal, AU1 (Cat. No. 690108) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated at RT for 1 h. The secondary antibody goat anti-mouse HRP was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

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