Name: anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1, 0.2 ml
Brand: PROGEN
SKU: 1NN1.1
Price: 110.31 EUR
Availability: InStock

anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1, 0.2 ml

Product Details

CAS number: 60-18-4

Chemical formulas: C9H11NO3

HS code: 30021500

Brand: PROGEN

anti-alpha-Smooth Muscle Actin, mouse monoclonal, 1A4/ASM-1, 100 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 100 μg/ml, monoclonal, affinity purified

1A4/ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (43 kDa). Tumors specificallly detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast. Tested cultured cell lines: stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

Host: mouse
Antibody type: monoclonal
Isotype: IgG2a
Clone: 1A4/ASM-1
Immunogen: synthetic N-terminus decapeptide of alpha-smooth-muscle isoform of actin
UniprotID: P62739 (Bovine), P08023 (chicken), P62736 (human), P62737 (mouse), P62738 (rat)
Synonym: actin, aortic smooth muscle, alpha-actin-2, cell growth-inhibiting gene 46 protein [cleaved into: actin, aortic smooth muscle, intermediate form], ACTA2, ACTSA, ACTVS, GIG46
Conjugate: unconjugated
Purification affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, chicken, horse, human, mouse, rat

Toepassing

Immunocytochemistry (ICC): Assay dependent
Immunohistochemistry (IHC) - frozen: 1:200-1:1,000 (100-500 ng/ml)
Immunohistochemistry (IHC) - paraffin: 1:200-1:1,000 (100-500 ng/ml, protease treatment and/or microwave treatment recommended)
Western Blot (WB): 1:1,000 (100 ng/ml)

Western blot analysis of human HeLa cell lysate with anti-alpha-Smooth Muscle Actin antibody. Western blot analysis was performed on either 10 μg, 5 μg or 2.5 μg of HeLa lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. No. 690001) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

IHC analysis of head and neck squamous cell carcinoma using anti-alpha-Smooth Muscle Actin antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. No. 690001) was diluted in PBS (antibody concentration 200 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).

IHC analysis of human colon using anti-alpha-Smooth Muscle Actin antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. No. 690001) was diluted in PBS (antibody concentration 400 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).

Geen medisch hulpmiddel / Geen ivD-product

Clonality	Monoclonal
Concentration	100 µg/ml
Raised in	Hybridoma cell culture supernatant
Immunglobulin (Ig) class	IgG2a
Clone	1A4/ASM-1
Conjugate	Unconjugated
Purification	Affinity chromatography
Purity (SDS-PAGE)	≥95 %
PBS buffer, pH 7.4 with 0.09 % sodium azide and 0.5 % BSA., Short term at 2-8 °C (+4 °C); long term storage in aliquots at -20 °C; avoid freeze/thaw cycles.