Name: anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, 0.2 ml
Brand: PROGEN
SKU: 1NLN.1
Price: 110.31 EUR
Availability: InStock

anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, 0.2 ml

Product Details

HS code: 30021500

Brand: PROGEN

anti-Cardiac Actin mouse, monoclonal, AC1-20.4.2, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 50 μg/ml, monoclonal, affinity purified

Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.

Polypeptide reacting: specific for fetal (cardiac) isoform of actin.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: AC1-20.4.2
Immunogen: synthetic NH2 terminus decapeptide of cardiac isoform of actin
UniprotID: P68034 (chicken), P68032 (human), G1STB6 (rabbit)
Synonym: actin, alpha cardiac muscle 1, alpha-cardiac actin [cleaved into: actin, alpha cardiac muscle 1, intermediate form], ACTC1, ACTC
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: bovine, chicken, human, mouse, rabbit

Toepassing

Immunohistochemistry (IHC) - frozen: 1:10 (5 μg/ml; include 0.5 M NaCl in all washing buffers to enhance specificity)
Immunohistochemistry (IHC) - paraffin: 1:10 (5 μg/ml; microwave treatment recommended)
Western Blot (WB): 1:1,000 (50 ng/ml; include 1 M NaCl in all washing buffers to enhance specificity)

Western blot analysis of human heart whole tissue lysate and with anti-Cardiac Actin antibody. Western blot analysis was performed on 20 μg human heart lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 (Cat. No. 690075) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, liquid, purified

Geen medisch hulpmiddel / Geen ivD-product

Clonality	Monoclonal
Concentration	50 µg/ml
Raised in	Hybridoma cell culture supernatant
Immunglobulin (Ig) class	IgG1
Clone	AC1-20.4.2
Conjugate	Unconjugated
Purification	Affinity chromatography
Purity (SDS-PAGE)	≥95 %
PBS buffer, pH 7.4 with 0.09 % sodium azide and 0.5 % BSA., Short term at 2-8 °C (+4 °C); long term storage in aliquots at -20 °C; avoid freeze/thaw cycles.