Home/Store/Life Science/Biochemie/Western & ELISA
anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24, 0.2 ml

anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24, 0.2 ml

SKU 1NP8.1
€ 110,31
In stock
1
Save this product for later
anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24, 0.2 ml
Product Details
HS code: 30021500
Brand: PROGEN
anti-Glial Fibrillary Acidic Protein, mouse monoclonal, GF 12.24, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24 50 μg/ml, monoclonal, affinity purified

GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects.

Polypeptide reacting: Mr 50,000 glial filament protein GFAP (Glial Fibrillary Acidic Protein, Glial Filament Protein).

Tumors specifically reacting: astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas.

Reactivity on cultured cell lines: human U 333CG/343MG

Host: mouse
Antibody type: monoclonal
Isotype: IgG2a
Clone: GF 12.24
Immunogen: intermediate filament cytoskeleton from cultured human glioma cells
UniprotID: Q28115 (bovine),P14136 (human),P03995 (mouse), P47819 (rat)
Synonym: glial fibrillary acidic protein, GFAP, glial Filament Protein, GFP
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, human, mouse, rat


Toepassing

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:100-1:200 (250-500 ng/ml)
Immunohistochemistry (IHC) - paraffin: 1:100-1:200 (250-500 ng/ml; microwave treatment recommended)
Western Blot (WB): assay dependent


1NP8_01
IHC analysis of mous brain using anti-GFAP antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24 (Cat. No. 690011) was diluted in PBS (antibody concentration 250 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
1NP8_02
IHC analysis of rat brain using anti-GFAP antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24 (Cat. No. 690011) was diluted in PBS (antibody concentration 250 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).


Geen medisch hulpmiddel / Geen ivD-product
Show More
You May Also Like
7-fluoro-2-methylquinoline-4-carboxylic acid, 95.0%, 1g
7-fluoro-2-methylquinoline-4-carboxylic acid, 95.0%, 1g
7-fluoro-2-methylquinoline-4-carboxylic acid, 95.0%, 1g
SKU F308396-1G
€ 132,00
Boc-Asn(Trt)-OH, 95%, 25g
Boc-Asn(Trt)-OH, 95%, 25g
Boc-Asn(Trt)-OH, 95%, 25g
SKU M03265-25G
€ 36,30
3-[(2,2,2-trifluoroethoxy)methyl]benzonitrile, 97%, 25g
3-[(2,2,2-trifluoroethoxy)methyl]benzonitrile, 97%, 25g
3-[(2,2,2-trifluoroethoxy)methyl]benzonitrile, 97%, 25g
SKU F428755-25G
€ 745,80
N,N,N',N'-Tetramethylfluoroformamidinium hexafluorophosphate, 98.0%, 5g
N,N,N',N'-Tetramethylfluoroformamidinium hexafluorophosphate, 98.0%, 5g
N,N,N',N'-Tetramethylfluoroformamidinium hexafluorophosphate, 98.0%, 5g
SKU F024896-5G
€ 17,60
2,3-Bis(hydroxymethyl)naphthalene>98.0%(GC)1g
2,3-Bis(hydroxymethyl)naphthalene>98.0%(GC)1g
2,3-Bis(hydroxymethyl)naphthalene>98.0%(GC)1g
SKU B1589-1G
€ 229,90
Tamper-evident closure PP blue GL 54
Tamper-evident closure PP blue GL 54
Tamper-evident closure PP blue GL 54
SKU 8-1110-54-BL
€ 0,93
tert-Butylphenylcarbonate, 95.0%, 500g
tert-Butylphenylcarbonate, 95.0%, 500g
tert-Butylphenylcarbonate, 95.0%, 500g
SKU F044604-500G
€ 244,20
2,4,6-Trichloro-5-methoxypyrimidine, 95.0%, 100mg
2,4,6-Trichloro-5-methoxypyrimidine, 95.0%, 100mg
2,4,6-Trichloro-5-methoxypyrimidine, 95.0%, 100mg
SKU F320580-100MG
€ 15,40
4-Methyl-1-nitro-2-(trifluoromethyl)benzene, 95.0%, 25g
4-Methyl-1-nitro-2-(trifluoromethyl)benzene, 95.0%, 25g
4-Methyl-1-nitro-2-(trifluoromethyl)benzene, 95.0%, 25g
SKU F216630-25G
€ 1 075,80
Pentafluorobenzyl chloride, 95.0%, 25g
Pentafluorobenzyl chloride, 95.0%, 25g
Pentafluorobenzyl chloride, 95.0%, 25g
SKU F009308-25G
€ 492,80
Citral (cis- and trans- mixture)>96.0%(GC)25mL
Citral (cis- and trans- mixture)>96.0%(GC)25mL
Citral (cis- and trans- mixture)>96.0%(GC)25mL
SKU D0762-25ML
€ 17,60
1-Methylguanidine Hydrochloride>98.0%(N)(T)5g
1-Methylguanidine Hydrochloride>98.0%(N)(T)5g
1-Methylguanidine Hydrochloride>98.0%(N)(T)5g
SKU M0336-5G
€ 22,00
  • Search Products
  • My Account
  • Track Orders
  • Favorites
  • Shopping Bag
Display prices in:EUR
Doorgaan naar hoofdinhoud
SciSupplies
Winkel
Over
Neem contact met ons op
FAQ
NL
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Mijn accountZoeken
Winkelwagen
Menu
Onderdeel van HD Beverwijk Trading
Taal:
NL
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Algemene voorwaardenPrivacybeleidVerzend- en betaalinformatieRetourbeleidRapporteer misbruik
Gemaakt met Ecwid by Lightspeed