anti-Keratin K18 mouse monoclonal, Ks18.04, 1 ml
SKU 1NLY.2
€ 286,44
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anti-Keratin K18 mouse monoclonal, Ks18.04, 1 ml
Product Details
HS code: 30021500
Brand: PROGEN
anti-Keratin K18 mouse, monoclonal, Ks18.04, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Keratin K18 mouse monoclonal, Ks18.04 50 μg/ml, monoclonal, affinity purified
Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide reacting: Mr 45,000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Tested cultured cell lines: MCF-7.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: Ks18.04
Immunogen: human keratin K18 from HeLa cytoskeletal preparation
UniprotID: A1XEA5 (Bovine),P05783 (human), P05784 (mouse), F1SGG1 (Pig),Q5BJY9 (rat), W5Q5M3 (sheep), W5Q5M3 (sheep)
Synonym: keratin, type I cytoskeletal 18, cell proliferation-inducing gene 46 protein, cytokeratin-18, CK-18, keratin-18, K18, KRT18, CYK18, PIG46
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish
Toepassing
Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:20-1:200
Immunohistochemistry (IHC) - paraffin: 1:20-1:200 (microwave treatment recommended)
Western Blot (WB): 1:100-1:500 (0.5 μg/ml-0.1 μg/ml)
Western blot analysis of human MCF-7 cell lysate with anti-Keratin K18 antibody. Western blot analysis was performed on 10 μg or 5 μg of MCF-7 lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Keratin K18 mouse monoclonal, Ks18.04 (Cat. No. 690028) was diluted in blocking buffer (antibody concentration 0.25 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg)
Geen medisch hulpmiddel / Geen ivD-product
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