anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, 0.2 ml

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia.

Polypeptide reacting: Mr 52,500, Mr 58,000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain of Keratin K8 (Waseem et al., 2004).

Reactivity on cultured cell lines: MCF-7, RT 112, HT-29, HaCaT, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.

Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: Ks 5+8.22/C22
Immunogen: human keratin K8, purified from SDS PAGE gel
UniprotID: Q5XQN5 (bovine), Q7RTS7 (human), Q922U2 (mouse), P05786 (bovine), P05787 (human), P11679 (mouse)
Synonym: keratin, type II cytoskeletal 74, cytokeratin-74, CK-74, keratin-5c, K5C, keratin-74, K74, type II inner root sheath-specific keratin-K6irs4, type-II keratin Kb37, KRT74, K6IRS4, KB37, KRT5C, KRT6IRS4, keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, keratin-8, K8, type-II keratin Kb8, KRT8, CYK8
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, human, mouse, rat

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, protease treatment and/or microwave treatment recommended)
Western Blot (WB): 1:500 (0.1 μg/ml)