Name: anti-6-His-tag mouse monoclonal, 6His, 100 µg
Brand: PROGEN
SKU: 1NNA.2
Price: 454.37 EUR
Availability: InStock

anti-6-His-tag mouse monoclonal, 6His, 100 µg

Product Details

P-phrases: P260 P270 P280 P302+P352 P305+P351+P338 P310

H-phrases: H302+H312+H332-H314-H412 EUH032

CAS number: 593-84-0

Chemical formulas: C2H6N4S

Hazard class: 8 II

HS code: 30021500

Brand: PROGEN

anti-6-His-tag mouse, monoclonal, 6His, after dissolv. in 400 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
High affinity to the respective protein tag
Robust & highly specific detection of the fusion protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-6-His-tag mouse monoclonal, 6His lyophilized, monoclonal, affinity purified

The monoclonal 6-His antibody recognizes polyhistidine (6-His). The 6-His tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: 6His
Immunogen: recombinant protein containing the sequence HHHHHH
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IP, WB
Reactivity: 6-His

Toepassing

Immunocytochemistry (ICC): assay dependent
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:2,000-1:5,000 (0.125-0.05 μg/ml)

Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 160 ng, 80 ng or 20 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.125 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 50 ng or 25 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

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