anti-DNA mouse monoclonal, AC-30-10, 0.2 ml
SKU 1NN5.1
€ 132,31
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anti-DNA mouse monoclonal, AC-30-10, 0.2 ml
Product Details
P-phrases: P280 P302+P352 P304+P340 P305+P351+P338
H-phrases: H315-H319-H335
CAS number: 15163-36-7
Chemical formulas: C15H33NO3S
HS code: 30021500
Brand: PROGEN
anti-DNA mouse monoclonal,, AC-30-10, 100 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-DNA mouse monoclonal, AC-30-10 100 μg/ml, monoclonal, chromatographically purified
AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for the detection of mycoplasma contamination in cell cultures.
Binding characteristics: reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA.
Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.
Host: mouse
Antibody type: monoclonal
Isotype: IgM
Clone: AC-30-10
Immunogen: double- and single-stranded DNA
Conjugate: unconjugated
Purification: size exclusion chromatography
Intended use: research use only
Application: dot blot, ICC/IF, IHC
Reactivity: all species
Toepassing
Dot Blot: Assay dependent (on nitrocellulose membrane, after baking at 70°C)
Immunocytochemistry (ICC): 1:200 (0.5 μg/ml)
Immunohistochemistry (IHC) - frozen: 1:10 (10 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10 (10 μg/ml, microwave treatment recommended)
Immunofluorescence analysis of HeLa cells with anti-DNA antibody. Fixation was performed using 3% paraformaldehyde for 10 min at RT. Cells were blocked with 5% BSA in PBST (PBS + 0.1% Tween 20) for 1 h at RT and permeabilized with 0.3% Triton-X 100 in PBS for 10 min at RT. The primary antibody anti-DNA mouse monoclonal, AC-30-10 (Cat. No. 690014) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated over-night at 4°C. The secondary antibody goat anti-mouse IgM Alexa488 was also diluted in blocking buffer (antibody concentration 2.5 μg/ml) and incubated for 30 min at 37°C and 30 min at RT. Nuclear DNA was stained with DAPI in blue.
Geen medisch hulpmiddel / Geen ivD-product
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