HPLC column NUCLEODUR® NH2 5 µm, 250 mm, 4 mm, 1 Piece(s)

This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR^® silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.

Applications:
Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases), USP L8, suitable for LC/MS.



Similar phases:
NUCLEOSIL^® NH₂ / NH₂-RP

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!

For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.

Further HPLC columns from Macherey-Nagel available on request.

The ideal phase for overall sophisticated analytical separations

This high density phase with its endcapping suppresses any unwanted polar interactions between the silica surface and the sample, which makes “Gravity” particularly suitable for the separation of basic and other ionizable analytes.

Properties:

• Suitable for LC/MS and HPLC at pH extremes (pH 1-11)
• Ideal for method development
• Superior base deactivation
• Multi-Endcapping

Recommended application(s):

• In general, compounds with ionisable functional groups such as basic pharmaceuticals and pesticides e.g. analgesics, anti-inflammatories, antidepressants, herbicides, phytopharmaceuticals, immunosuppressants.
• USP L1 (C₁₈) · USP L7 (C₈)