Magnesium chloride solution, 1 ml, 2 x 0,5 ml in tubes
SKU 1HY7.1
€ 14,39
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Magnesium chloride solution, 1 ml, 2 x 0,5 ml in tubes
Product Details
CAS number: 5794-13-8
Chemical formulas: C4H8N2O3 · H2O
HS code: 28273100
Brand: Carl Roth
Magnesium chloride solution, 25 mM, for PCR and molecular biology, Molar mass (M) 95,22 g/mol, Density (D) 1,003 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C, Empirical formula MgCl2
Steriel
Magnesium chloride solution 25 mM, sterile, for molecular biology
In a PCR reaction, cofactors such as divalent cations (MgCl2) are needed for an optimal process. Magnesium interacts with the DNA template, dNTPs and the polymerase in a PCR reaction. The MgCl2 concentration influences the productivity and accuracy of the polymerase. It also influences the binding of the primers to the template.
Toepassingsgebieden:
- Too high magnesium concentration can lead to reduced specificity and undesired PCR products
- Too low a magnesium concentration can lead to no PCR product at all
- Magnesium influences the melting point of double-stranded DNA
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