Name: Transfer membrane ROTI®PVDF 0.45
Brand: Ratiolab GmbH
SKU: 200T.1
Price: 548.69 EUR
Availability: InStock

Transfer membrane ROTI®PVDF 0.45

Product Details

P-phrases: P210 P260 P280 P301+P310 P303+P361+P353 P305+P351+P338

H-phrases: H226-H304-H312+H332-H315-H319-H335-H373

CAS number: 1330-20-7

Chemical formulas: C8H10

Hazard class: 3 III

HS code: 39219090

Material: PVDF

Brand: Ratiolab GmbH

ROTI®PVDF, pore size 0.45 µm, Roll, 300 x 30 cm, PVDF Membrane

Materiaal: polyvinylidenfluoride (PVDF).

PVDF membrane for protein analyses.

ROTI^®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.

Gebruiksinstructies

The membrane shows superior protein binding parameters and stands out for its particularly low background. It may also be used very well after repeated stripping and harsh washing processes.
This mechanically rigid transfer membrane can be easily cut to the desired gel size and it remains flexible and tear-proof even when dry.
Briefly immerse the membrane membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every blotting system.
Due to production processes, the membrane shows two different sides - one smooth and one raw. For optimally distinct bands, please use the smooth side for protein binding.

Transfer membrane ROTI^®PVDF 0.45

Geschikt voor

Optimal for proteins≥20 kDa.
Detection by Chemoluminescence, radioactivity, colour.

Toepassingsgebieden:

Western blotting
Immuno-blotting
Solid phase assays
Amino acid or protein analysis

Geen medisch hulpmiddel / Geen ivD-product

Technische informatie

Afmeting	300 × 30 cm
Poriëngr.	0,45 µm
Binding	hydrophobic
Bindingscapaciteit	125 µg/cm²
Dikte	190±50 µm
Immobilisering	not necessary

Technical Parameters of ROTH-Transfer Membranes

Membrane	Material	Characteristics	Thickness (μm)	Binding capacity (μg/cm²)	Pore size (μm)	Immobilisation
Roti^® Fluoro PVDF	Polyvinyliden fluoride	hydrophobic	125 ±25	300	0,2	not necessary
Roti^® PVDF 0.2	Polyvinyliden fluoride	hydrophobic	140 ±20	≥125	0,2	not necessary
Roti^® PVDF	Polyvinyliden fluoride	hydrophobic	125 ±25	ca. 200	0,45	not necessary
Immobilon P	Polyvinyliden fluoride	hydrophobic	120 ±25	150-300	0,45	not necessary
Roti^® Nylon 0.2	Nylon 6,6	neutral/amphoteric	150 ±20	500	0,2/0,45	UV, baking
Roti^® Nylon plus	Nylon 6,6	hydrophilic, positively charged	160 ±20	500	0,45	not necessary, UV and baking possible
Roti^® NC	Nitrocellulose	electrostatic, hydrophobic	150 ±50	125-200	0,2	UV, baking (max. 80 °C)
Roti^® NC 0.45	Nitrocellulose	electrostatic, hydrophobic	150 ±10	≥125	0,45	UV, baking (max. 80 °C)
Amersham Protran^®	Nitrocellulose	electrostatic, hydrophobic	unknown	11-72	0,2/0,45	UV, baking (max. 80 °C)
Amersham Protran^® supported	Nitrocellulose	electrostatic, hydrophobic	unknown	86-110	0,2/0,45	UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores	Size (kD)
genomic DNA (human)	>11000000
E. coli	approx. 1500000
0.45 μm	approx. 5000
0.2 μm	approx. 2000
DNA 3 kb	approx. 1000
RNA 1 kb	approx. 300
Antibodies (IgG)	approx. 150
Proteins in the lab	approx. 10–300
BSA (Albumin)	69

Application of ROTH-Transfer Membranes

Biomolecules	Nylon plus	Nylon 0.2	PVDF 0.2	PVDF	Fluoro PVDF	NC	NC 0.45
DNA	+++	+	-	-	-	+	+
RNA	+	+++	-	-	-	+	+
Proteins	-	+	+++	+++	+++	+	+
Detection System
Colour	+++	+++	+++	+++	+++	+++	+++
Radioactivity	+++	+++	+++	+++	+++	+++	+++
Chemoluminescence	+++	+++	+++	+++	-	+	+
Fluorescence	+++	+++	-	-	+++	-	-
+++: recommended		+: suitable		-: not