ROTI®Plate90 TSA 4N

Sterile ready-to-use culture media plates for microbiological analysis.

ROTI^®Plate90 are prepared culture media plates with a diameter of 90 mm. They are thus adapted to the size of membrane filters for assaying of low-germ fluids by membrane filtration method. They are also applicable for the microbiological analysis of samples in general.

The germs of expected low-germ liquid samples are concentrated by membrane filtration. Afterwards, the membrane is placed on top of the medium and incubated. Using a membrane with a grid helps counting the resulting colonies.
Our products are tested and approved exclusively for research and development.

Samples which are expected to have a higher microbiological burden are prepared in a serial dilution. Afterwards, every dilution step is plated and incubated. The number of grown colonies is noted as result.

Tryptone soy agar (TSA) is recommended by the Pharmacopeia Europaea for testing products under investigation for the presence of bacteria by the membrane filter method, and for counting bacteria on agar plates or by the dilution method (MPN method). As this medium contains two peptones obtained by enzymatic hydrolysis of casein and soy protein, it supports a wide range of microorganisms, including fastidious aerobic and anaerobic organisms.
The included disinhibitors lecithin, polysorbate 80 (Tween^®80), sodium thiosulphate and histidine are also called neutralisers. Disinhibitors serve to neutralise residues of disinfectants and preservatives and ensure bacterial growth.

These plates are triple wrapped, which allows the removal of each individual package when the plates are taken further into the clean room.