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ROTI®Pol TaqS, 500 µl, 5 x 100 µl

ROTI®Pol TaqS, 500 µl, 5 x 100 µl

SKU 9223.2
€ 401,46
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ROTI®Pol TaqS, 500 µl, 5 x 100 µl
Product Details
HS code: 38229000
Brand: Carl Roth
ROTI®Pol TaqS (5 x 100 µl), 5 U/µl, Storage temp. -20 °C

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol TaqS 5 U/μl

Recombinant full-length form of the heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer, plus additional 10x concentrated PCR reaction buffer and 10x concentrated PCR reaction buffer with red gel loading dye. ROTI®Pol TaqS is recommended for all standard PCR applications.

This polymerase set ROTI®Pol TaqS is the optimal choice for all standard Taq-based cycling protocols, as being performed in, for instance, analysis of cloning efficiency, routine screening processes, educational assays and much more. In combination with our unique buffers, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates.

ROTI®Pol TaqS is able to amplify PCR products up to 3 kb with genomic DNA and is appropriate for use in the amplification of DNA from genomic, viral, and plasmid templates. The TaqS DNA polymerase included in the set possesses a 5' → 3' polymerase- as well as a 5’-flap endonuclease activity, and generates a 3'dA (adenine)-overhang which may well be used for TA-cloning purposes.

RotiPol-Sensitivitaetsassay
Figure: Sensitivity assay using ROTI®Pol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.
M: 100 bp-DNA Ladder extended. NTC: no template control.

Filled in colour coded tubes, the set contains the DNA polymerase and two 10x concentrated reaction buffers with 20 mM MgCl2, one of which has been specially designed for direct gel loading following the PCR reaction. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The use of the colourless PCR reaction buffer is adequate for all general PCR applications and is particularly recommended when direct fluorescence or absorbance readings are required.


De set bevat

TaqS polymerase in storage buffer containing 50 % glycerol, PCR buffer (10x) with 20 mM MgCl2, PCR buffer red (10x) with 20 mM MgCl2 and 0,1 % cresol red.
Colour coded tubes.
Contents of this set may not be bought separately.



Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Toepassing Standard PCR
Amplicon-einden 3'dA
Polymerase Standard Taq polymerase
Reactiebuffer colourless + red (ready to load)
Toepassing Standard PCR

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

• Oxidoreductases (catalyse redox reactions)

• Transferases (transfer functional groups among substrates)

• Hydrolases (cleave bonds via addition of water)

• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

• Isomerases (transform chemical isomers)

• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Purity (enzyme) ≥98%
Enzyme activity 5 U/µl
Endonuclease activity none detected
Exonuclease activity none detected
Suitability for PCR (400 bp) complies
Suitability for PCR (3 kb) complies
Selective specificity in PCR complies
Stability (in storage buffer) complies
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