10fold stock solution tris buffered saline with Tween 20, pH 7,4.
Extremely versatile standard solution for protein biochemical detection systems, cytological staining and molecular-biological assays with similar fields of application as ROTI®Stock TBS.
1 x TBST is, for instance, used as basic solution for protein detection systems like Western-Blot analyses and Far-Western assays, for immunocytological and immunohistological assays, as well as for whole mount in situ hybridisations, apoptosis detection systems and staining of nuclei.
ROTI®Stock TBST can be used directly instead of TBS when the presence of Tween 20 is required in the detection system.
pHwaarde | 7,4 ±0,25 |
1.5 M NaCl, 30 mM KCl, 250 mM Tris, 0.5 % Tween® 20 in deionised water, pH value 7.4 ±0.25, 0.2 μm filtered and steam sterilised. |