Name: ROTIPHORESE®Sequencing gel buffer concentrate
Brand: Carl Roth
SKU: 3050.1
Price: 35.1 EUR
Availability: InStock

ROTIPHORESE®Sequencing gel buffer concentrate

Product Details

HS code: 38229000

Brand: Carl Roth

ROTIPHORESE® Sequencing gel, buffer concentrate,, 50 % urea (w/v, 8.3 M) in 10x TBE buffer sol., ready-to-use, Density (D) 1,185 g/cm³, Boiling point (bp) 100 °C, Storage temp. +4 to +15 °C

For preparation of 4-20 % sequencing gels
Most simple handling of three optimised ready-to-use solutions
No tedious dissolving of urea
Prevents acrylamide dusts
Reproducible, superior quality of gels
In tried-and-tested ROTIPHORESE^® quality

Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE^® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.
In addition, the ROTIPHORESE^®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE^®Sequencing gel buffer concentrate and ROTIPHORESE^®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE^®10x TBE buffer or water, the urea content can be reduced to up to 40 %.

Gebruiksinstructies

Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28).

ROTIPHORESE^®Sequencing gel buffer concentrate ROTIPHORESE^® 50 % (w/v, 8,3 M) in 10x TBE

Ready-to-use 10x TBE stock solution (1 M Tris borate, 20 mM EDTA buffer, pH 8,3) with 50 % urea .
For the preparation of polyacrylamide sequencing gels with 40-50 % urea (6,7-8,3 M).

Also part of the ROTIPHORESE^®DNA sequencing system (Art. No. A431.1).

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE^® ready-to-use solution.

Superior quality
ROTIPHORESE^® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE^®-solutions, each batch undergoes electrophoretic testing.

The right choice for each and every application
The large variety of different ROTIPHORESE^® solutions provides you with the right product for each and every application. ROTIPHORESE^® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE^® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.

ROTIPHORESE^®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE^® ready-to-use solutions can be used directly.

Recommendation for Use of ROTIPHORESE^® Ready-Made Gel Solutions

Analysis of	Gel solution	Art. No.
Proteins/peptides >15 kDa, DNA fragments	Gel 30 (37,5:1)	3029.1
Proteins/peptides >15 kDa, DNA fragments	Gel 40 (37,5:1)	T802.1
Proteins/peptides <30 kDa and DNA oligonucleotides	Gel 40 (29:1)	A515.1
DNA sequences (manual sequencing)	ROTIPHORESE^®DNA sequencing system	A431.1
DNA sequences (manual sequencing)	Gel 40 (19:1)	3030.1
DNA sequences (short-run automat. sequencing)	NF solution 40 % (19:1)	A516.1
DNA sequences (long-run automat. sequencing)	NF solution 30 % (29:1)	A124.1
DNA sequences (long-run automat. sequencing)	NF solution 40 % (29:1)	A121.1

1.0 M TRIS borate (pH 8.3), 20 mM EDTA., 50 % urea., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).