Home/Store/Laboratory supplies/Filtration, water treatment, dialysis/Filtration and separation/Membrane filters
Transfer membrane Amersham Protran® NC 0.45 disks 2.5 cm, 100 Piece(s)

Transfer membrane Amersham Protran® NC 0.45 disks 2.5 cm, 100 Piece(s)

SKU 4589.1
€ 194,42
In stock
1
Save this product for later
Transfer membrane Amersham Protran® NC 0.45 disks 2.5 cm, 100 Piece(s)
Product Details
HS code: 39207990
EAN: 8721028170255
Brand: Cytiva
Material: nitrocellulose (100 %).

Transfer membrane for most nucleic acid and protein immobilising techniques

Replaces Protran® BA 85 from Whatman®

Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background.


Directions for use

Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer.
Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).


Application examples

Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.
Detection systems are radioactivity, chemoluminescence, colour



Transfer membrane Amersham™ Protran® NC 0.45 disks Ø2.5 cm

For colony and plaque lifting in small petri dishes.



Technical Information
Dimensions Ø2.5 cm
Version disks
Pore size 0,45 µm
Type Protran® NC 0,45
Bond electrostatic, hydrophobic
Binding capacity 86-110 µg/cm²
Immobilisation UV, baking (max. 80 °C)

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Show More
You May Also Like
tert-Butyl (2-amino-3-methylbutyl)carbamate, 95%, 250mg
tert-Butyl (2-amino-3-methylbutyl)carbamate, 95%, 250mg
tert-Butyl (2-amino-3-methylbutyl)carbamate, 95%, 250mg
SKU F762381-250MG
€ 346,50
3-Fluoro-5-hydroxypicolinic acid, 97%, 100mg
3-Fluoro-5-hydroxypicolinic acid, 97%, 100mg
3-Fluoro-5-hydroxypicolinic acid, 97%, 100mg
SKU F762502-100MG
€ 480,70
2-(6-BROMOPYRIDIN-3-YL)ACETIC ACID, 95.0%, 5g
2-(6-BROMOPYRIDIN-3-YL)ACETIC ACID, 95.0%, 5g
2-(6-BROMOPYRIDIN-3-YL)ACETIC ACID, 95.0%, 5g
SKU F539939-5G
€ 1 749,00
(4-(Isopentyloxy)phenyl)methanamine, 98%, 250mg
(4-(Isopentyloxy)phenyl)methanamine, 98%, 250mg
(4-(Isopentyloxy)phenyl)methanamine, 98%, 250mg
SKU F682566-250MG
€ 244,20
8-Bromo-5-fluoro-1,2,3,4-tetrahydroisoquinoline, 95%, 500mg
8-Bromo-5-fluoro-1,2,3,4-tetrahydroisoquinoline, 95%, 500mg
8-Bromo-5-fluoro-1,2,3,4-tetrahydroisoquinoline, 95%, 500mg
SKU F760779-500MG
€ 796,40
2-Bromo-5-chloro-3-methylpyrazine, 95.0%, 100mg
2-Bromo-5-chloro-3-methylpyrazine, 95.0%, 100mg
2-Bromo-5-chloro-3-methylpyrazine, 95.0%, 100mg
SKU F238708-100MG
€ 74,80
tert-Butyl 6-methoxy-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indole-1-carboxylate, 95.0%, 100mg
tert-Butyl 6-methoxy-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indole-1-carboxylate, 95.0%, 100mg
tert-Butyl 6-methoxy-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indole-1-carboxylate, 95.0%, 100mg
SKU F213712-100MG
€ 160,60
Squalane>98.0%(GC)25mL
Squalane>98.0%(GC)25mL
Squalane>98.0%(GC)25mL
SKU H0096-25ML
€ 18,70
Benzyl Cinnamate>98.0%(GC)25g
Benzyl Cinnamate>98.0%(GC)25g
Benzyl Cinnamate>98.0%(GC)25g
SKU C0358-25G
€ 17,60
4-(4,5-DIHYDRO-2-OXAZOLYL)-PHENOL, 97.0%, 1g
4-(4,5-DIHYDRO-2-OXAZOLYL)-PHENOL, 97.0%, 1g
4-(4,5-DIHYDRO-2-OXAZOLYL)-PHENOL, 97.0%, 1g
SKU F534804-1G
€ 500,50
n-Methoxy-n-methyl-4-(trifluoromethoxy)benzamide, 97%, 250mg
n-Methoxy-n-methyl-4-(trifluoromethoxy)benzamide, 97%, 250mg
n-Methoxy-n-methyl-4-(trifluoromethoxy)benzamide, 97%, 250mg
SKU F649926-250MG
€ 699,60
1-(4-Bromonaphthalen-1-yl)ethanone, 95.0%, 250mg
1-(4-Bromonaphthalen-1-yl)ethanone, 95.0%, 250mg
1-(4-Bromonaphthalen-1-yl)ethanone, 95.0%, 250mg
SKU F329850-250MG
€ 22,00
  • Search Products
  • My Account
  • Track Orders
  • Favorites
  • Shopping Bag
Display prices in:EUR
Skip to main content
SciSupplies
Store
About
Contact us
FAQ
EN
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
My accountSearch
Cart
Menu
Part of HD Beverwijk Trading
Language:
EN
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Terms & ConditionsPrivacy PolicyShipping & Payment InfoReturn PolicyReport abuse
Made with Ecwid by Lightspeed