Home/Store/Laboratory supplies/Filtration, water treatment, dialysis/Filtration and separation/Membrane filters
Transfer membrane ROTI®Nylon 0.2, 300 × 30 cm, 1 Piece(s)

Transfer membrane ROTI®Nylon 0.2, 300 × 30 cm, 1 Piece(s)

SKU AE50.1
€ 429,07
In stock
1
Save this product for later
Transfer membrane ROTI®Nylon 0.2, 300 × 30 cm, 1 Piece(s)
Product Details
HS code: 84219990
Material: Nylon
EAN: 8721028170408
Brand: Vacuubrand
Material: nylon 6.6.

.




Transfer membrane ROTI®Nylon 0.2

Ideal for DNA/RNA transfer, particularly for colony- and plaque-lifting, as well as for all multiple rehybridisations.
Especially recommended for Northern-blots.


ROTI®Nylon 0.2 is a neutral, enhanced Nylon membrane. Based on the material it provides a superior tensile strength and is extremely resistant against alcohol, acetone, and many other organic solvents.

In a buffer pH of 6,5 ROTI®Nylon 0.2 is uncharged and by means of the surface-standing amino- and carboxy-groups has superior hydrophilic qualities. The charge of the membrane (ζ-potential) may be altered through the pH of the buffer - the membrane is positively charged at pH <6,5 and negatively charged at pH >6,5.
Membrane of particulary high DNA-binding capacity. Available as roll (pore size 0,2 µm) for high-flexible usage, and as disks (pore size 0,45 µm) for colony- and plaque-lifting. May be steam sterilised.
Covalent bondage of transferred nucleic acids is achieved by cross linking (baking for 15 min at 80 °C or UV-cross linking for 20 sec at 260 nm). Due to the excellent signal-to-noise ratio of the membrane, very sensitive detection (single copy-gene detection) and multiple rehybridisation is possible without loosing sensitivity.
Detection systems: radioactivity, chemoluminescence, fluorescence

Application examples:
  • Southern- and Northern blot
  • Dot Blot
  • DNA fingerprinting
  • ELISA
  • colony and plaque lifting
  • Macro- and Microarrays
  • Multiple Rehybridisations


Technical Information
Dimensions 300 × 30 cm
Version roll
Pore size 0,2 µm
Type 0,2 µm
Load neutral/amphoteric
Binding capacity 500 µg/cm²
Thickn. 150±20 µm
Immobilisation UV, baking

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Show More
You May Also Like
(S)-3-(4-ACETYLPHENYL)-2-AMINOPROPANOIC ACID HCL, 95.0%, 25g
(S)-3-(4-ACETYLPHENYL)-2-AMINOPROPANOIC ACID HCL, 95.0%, 25g
(S)-3-(4-ACETYLPHENYL)-2-AMINOPROPANOIC ACID HCL, 95.0%, 25g
SKU F469985-25G
€ 609,40
Lanthanum chloride hydrate 99.99% 100 g
Lanthanum chloride hydrate 99.99% 100 g
Lanthanum chloride hydrate 99.99% 100 g
SKU 900915
€ 179,69
((2R,3S)-3-(tert-butyldiphenylsilyloxy)-2,3-dihydrofuran-2-yl)methanol, 98%, 50mg
((2R,3S)-3-(tert-butyldiphenylsilyloxy)-2,3-dihydrofuran-2-yl)methanol, 98%, 50mg
((2R,3S)-3-(tert-butyldiphenylsilyloxy)-2,3-dihydrofuran-2-yl)methanol, 98%, 50mg
SKU F788447-50MG
€ 181,50
Precision balance PNJ series Factory calibrated models, 0.1 g, 12000 g, PNJ 12000-1M
Precision balance PNJ series Factory calibrated models, 0.1 g, 12000 g, PNJ 12000-1M
Precision balance PNJ series Factory calibrated models, 0.1 g, 12000 g, PNJ 12000-1M
SKU ENH8.1
€ 1 040,04
2,3-Dimethyl-5H,6H,7H,8H-imidazo[1,2-a]pyridin-8-one, 97%, 1g
2,3-Dimethyl-5H,6H,7H,8H-imidazo[1,2-a]pyridin-8-one, 97%, 1g
2,3-Dimethyl-5H,6H,7H,8H-imidazo[1,2-a]pyridin-8-one, 97%, 1g
SKU F626043-1G
€ 414,70
2-(3-(trifluoromethyl)phenyl)-4,5-dihydrooxazole, 96%, 5g
2-(3-(trifluoromethyl)phenyl)-4,5-dihydrooxazole, 96%, 5g
2-(3-(trifluoromethyl)phenyl)-4,5-dihydrooxazole, 96%, 5g
SKU F608570-5G
€ 414,70
5-amino-2-isobutyl-1H-isoindole-1,3(2H)-dione, 95.0%, 1g
5-amino-2-isobutyl-1H-isoindole-1,3(2H)-dione, 95.0%, 1g
5-amino-2-isobutyl-1H-isoindole-1,3(2H)-dione, 95.0%, 1g
SKU F315320-1G
€ 135,30
Round filters ROTILABO® Type 112A, , 150 mm, 100 Piece(s)
Round filters ROTILABO® Type 112A, , 150 mm, 100 Piece(s)
Round filters ROTILABO® Type 112A, , 150 mm, 100 Piece(s)
SKU AP71.1
€ 22,90
N2-Methylpyridine-2,4-diamine hydrochloride, 95.0%, 5g
N2-Methylpyridine-2,4-diamine hydrochloride, 95.0%, 5g
N2-Methylpyridine-2,4-diamine hydrochloride, 95.0%, 5g
SKU F215212-5G
€ 1 679,70
4-Phenylazepane, 95.0%, 1g
4-Phenylazepane, 95.0%, 1g
4-Phenylazepane, 95.0%, 1g
SKU F228093-1G
€ 267,30
4-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)cyclohexanecarboxylic acid, 95.0%, 250mg
4-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)cyclohexanecarboxylic acid, 95.0%, 250mg
4-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)cyclohexanecarboxylic acid, 95.0%, 250mg
SKU F324175-250MG
€ 18,70
4-Chlorobenzo[d]isothiazol-3(2H)-one 1,1-dioxide, 98%, 250mg
4-Chlorobenzo[d]isothiazol-3(2H)-one 1,1-dioxide, 98%, 250mg
4-Chlorobenzo[d]isothiazol-3(2H)-one 1,1-dioxide, 98%, 250mg
SKU F606531-250MG
€ 814,00
  • Search Products
  • My Account
  • Track Orders
  • Favorites
  • Shopping Bag
Display prices in:EUR
Skip to main content
SciSupplies
Store
About
Contact us
FAQ
EN
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
My accountSearch
Cart
Menu
Part of HD Beverwijk Trading
Language:
EN
NLNederlands
DEDeutsch
ENEnglish
FRFrançais
ESSpanish
ITItaliano
Terms & ConditionsPrivacy PolicyShipping & Payment InfoReturn PolicyReport abuse
Made with Ecwid by Lightspeed