Name: anti-Synaptophysin mouse monoclonal, SY38, 1 ml
Brand: PROGEN
SKU: 1NN3.2
Price: 346.59 EUR
Availability: InStock

anti-Synaptophysin mouse monoclonal, SY38, 1 ml

Product Details

CAS number: 56-86-0

Chemical formulas: C5H9NO4

HS code: 30021500

Brand: PROGEN

anti-Synaptophysin mouse, monoclonal, SY38, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-Synaptophysin mouse monoclonal, SY38 50 μg/ml, monoclonal, affinity purified

SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas.

Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid.

Polypeptide reacting: 38 kDa transmembrane glycoprotein of presynaptic vesicles.

Tested cultured cell lines: rat PC-12 cell line.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: SY38
Immunogen: synaptophysin from presynaptic vesicles, prepared from bovine brain
UniprotID: P20488 (bovine), P08247 (human), Q62277 (mouse), P07825 (rat)
Synonym: synaptophysin, major synaptic vesicle protein p38, SYP
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, human, mouse, rat

Toepassing

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:50-1:200 (0.25-1 μg/ml, no protease treatment)
Immunohistochemistry (IHC) - paraffin: 1:50-1:200 (0.25-1 μg/ml, microwave treatment recommended, no protease treatment)
Western Blot (WB): 1:500-1:1,000 (0.05-0.1 μg/ml)

Mouse brain (courtesy of J. Heß, University Hospital Heidelberg)

Western blot analysis of mouse brain lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg mouse brain lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Western blot analysis of rat Ins-1 lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg rat Ins-1 lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

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