anti-p53 Protein mouse monoclonal, Bp53.11, 0.2 ml
SKU 1NNY.1
€ 110,31
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anti-p53 Protein mouse monoclonal, Bp53.11, 0.2 ml
Product Details
P-phrases: P261 P280 P302+P352 P333+P313
H-phrases: H317
HS code: 30021500
Brand: PROGEN
anti-p53 Protein mouse, monoclonal, Bp53.11, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-p53 Protein mouse monoclonal, Bp53.11 50 μg/ml, monoclonal, affinity purified
Excellent marker for wild-type and mutant forms of human p53 antigen.
Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31.
The antibody stains positively approx. 60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.
Reactivity on cultured cell lines: Hela, MCF-7.
Host: mouse
Antibody type: monoclonal
Isotype: IgG2a
Clone: Bp53.11
Immunogen: recombinant human p53 (transcription domain within the NH2 terminus)
UniprotID: Q96A56 (human)
Synonym: tumor protein p53-inducible nuclear protein 1, stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1, TP53INP1, P53DINP1, SIP
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: human
Toepassing
Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:1,000-1:3,000 (17-50 ng/ml)
Immunohistochemistry (IHC) - paraffin: 1:1,000-1:3,000 (17-50 ng/ml, microwave treatment recommended)
Western Blot (WB): 1:2,000-1:10,000 (5-25 ng/ml)
p53 staining in human squamous cell carcinoma (image courtesy of J. Heß, University Hospital Heidelberg)
p53 staining in human squamous cell carcinoma (image courtesy of J. Heß, University Hospital Heidelberg)
Western blot analysis of HEK lysate with anti-p53 antibody. Western blot analysis was performed on 20 μg HEK lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-p53, mouse monoclonal, Bp53.11 (Cat. No. 690039) was diluted in blocking buffer (antibody concentration 25 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
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