anti-6-His-tag mouse monoclonal, 6His, 25 µg
SKU 1NNA.1
€ 125,40
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anti-6-His-tag mouse monoclonal, 6His, 25 µg
Product Details
P-phrases: P260 P270 P280 P302+P352 P305+P351+P338 P310
H-phrases: H302+H312+H332-H314-H412 EUH032
CAS number: 593-84-0
Chemical formulas: C2H6N4S
Hazard class: 8 II
HS code: 30021500
Brand: PROGEN
anti-6-His-tag mouse, monoclonal, 6His, after dissolv. in 100 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-6-His-tag mouse monoclonal, 6His lyophilized, monoclonal, affinity purified
The monoclonal 6-His antibody recognizes polyhistidine (6-His). The 6-His tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: 6His
Immunogen: recombinant protein containing the sequence HHHHHH
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IP, WB
Reactivity: 6-His
Toepassing
Immunocytochemistry (ICC): assay dependent
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:2,000-1:5,000 (0.125-0.05 μg/ml)
Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 160 ng, 80 ng or 20 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.125 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 50 ng or 25 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
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