ROTI®Load 3 (LDS)

ROTI^®Load 1 (reducing) as well as ROTI^®Load 2 and 3 (non-reducing) represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI^®Load are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.

Modified, non-reducing protein sample-gel loading buffer for gels under denaturing or native conditions.

ROTI^®Load protein gel loading buffers are usually used as 1x concentrated solution. ROTI^®Load can either be added to the prepared sample or the precipitated proteins are dissolved directly into ROTI^®Load. Optionally, heat the ROTI^®Load mixed sample to 95 °C for 5 minutes to break hydrogen bonds and stretch the peptide chains.

During sample preparation and heating, ROTI^®Load 3 (LDS) efficiently prevents degradation of your proteins, also stabilising the pH value during the run.
This gel loading buffer is based on a triethanolamine buffered ready-to-use solution, in which the standard sodium dodecylsulphate (SDS) has been replaced by lithium dodecylsulphate (LDS). At low temperatures, LDS is much better soluble than SDS. Hence, no crystallisation of LDS takes place, and protein stability as well as band resolution is significantly enhanced.
Additionally, the formulation of ROTI^®Load 3 (LDS) has been optimised for ready-made gels, particularly Q-PAGE™ gels, but also ready-made gels of other standard suppliers.

For reducing conditions, ROTI^®Load 3 (LDS) may be supplemented with 20 µl/ml DTT solution (1 M) or with β-mercaptoethanol.